|
KMID : 1094720170220050512
|
|
Biotechnology and Bioprocess Engineering
2017 Volume.22 No. 5 p.512 ~ p.517
|
|
|
Biological characterization of epigallocatechin gallate complex with different steviol glucosides
|
|
Nguyen Thi Thanh Hanh
Kim Na-Hyun M.
Yeom Su-Cheong
Han Song-Hee
Kwak So-Hyung
Kim Seong-Bo
Park Jun-Seong
Mok Il-Kyoon
Kim Do-Man
|
|
|
Abstract
|
|
|
|
Steviol glucosides (SGs) such as rubusoside (Ru), stevioside (Ste), rebaudioside A (RebA) and stevioside glucosides (SG) are herbal tea sweeteners that enhance the solubility and stability of a number of pharmaceutically important compounds. The complex of epigallocatechin gallate (EGCG) with 10% (w/v) each Ru, Ste, RebA or SG enhanced the water solubility of EGCG over 15 times to 345, 312, 341, or 320 mg/mL, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of EGCG, EGCG-Ru, EGCG-Ste, EGCG-RebA, and EGCG-SG in water were 5.88, 6.03, 6.52, 4.89, and 4.23 μg/mL, respectively. EGCGs complexed with different SGs maintained inhibitory activities against human intestinal maltase, human pancreatic α-amylase, and the growth of Streptococcus mutans, Helicobacter pylori, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Clostridium difficile. In glucose tolerance test using C57BL/6 mice, plasma glucose levels in mice treated with EGCG or EGCG-Ste complex were decreased by 9.34%, which was 31.08% lower than those treated with maltose. The efficient and cost-effective EGCG-SGs production method might be applicable to produce water soluble bioactive nutraceuticals in large scale.
|
|
|
KEYWORD
|
|
|
epigallocatechin gallate, rubusoside, steviol glucosides, DPPH radical scavenging activity, solubilization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|